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KMID : 0364820120480010001
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Korean Journal of Microbiology
2012 Volume.48 No. 1 p.1 ~ p.7
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Interactions between Biosynthetic Pathway and Productivity of IAA in Some Rhizobacteria
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Kim Woon-Jin
Song Hong-Gyu
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Abstract
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This study explores the interaction between the production of indole-3-acetic acid (IAA), a typical phytohormone
auxin and the role of IAA biosynthetic pathways in each IAA producing rhizobacterial strain. The bacterial strains
were isolated from rhizosphere of wild plants and identified as Acinetobacter guillouiae SW5, Bacillus thuringiensis
SW17, Rhodococcus equi SW9, and Lysinibacillus fusiformis SW13. A. guillouiae SW5 exhibited the highest
production of IAA using tryptophan-dependent pathways among the 4 strains. When indole-3-acetamide (IAM) was
added, Rhodococcus equi SW9 showed the highest IAA production of 3824 μg/mg protein using amidase activity. A.
guillouiae SW5 also showed the highest production of IAA using two pathways with indole-3-acetonitrile (IAN),
and its nitrile hydratase activity might be higher than nitrilase. B. thuringiensis SW17 showed the lowest IAA
production, and most of IAA might be produced by the amidase activity, although the nitrilase activity was the
highest among 4 strains. The roles of nitrile converting enzymes were relatively similar in IAA synthesis by
Lysinibacillus fusiformis SW13. Tryptophan-independent pathway of IAA production was utilized by only A.
guillouiae SW5.
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KEYWORD
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indole acetic acid, nitrilase, nitrile converting enzyme, rhizobacteria
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